South Asian Journal of Engineering and Technology

Vol. 8 No. 1 (2019): Volume 8, Issue 1, Year 2019
Articles

Isolation and characterization of mitochondria and lysosome from isoproterenol induced cardiotoxic rats

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  • Malathi Mangalanathan,
  • Tamiloli Devendhiran,
  • Saraswathi Uthamaramasamy,
  • Keerthika Kumarasamy,
  • K Mohanraj,
  • Kannagi Devendhiran,
  • Saroj Adhikari,
  • Mei Ching Lin
Malathi Mangalanathan
Department of Biochemistry, PSG College of Arts & Science, Coimbatore- 641014, Tamilnadu, India
Tamiloli Devendhiran
Department and Graduate Institute of Applied Chemistry, Chaoyang University of Technology, No.16, Jifeng East Road, Wufeng District, Taichung City – 41349, Taiwan (R.O.C).
Saraswathi Uthamaramasamy
Department of Biochemistry, PSG College of Arts & Science, Coimbatore- 641014, Tamilnadu, India.
Keerthika Kumarasamy
Department and Graduate Institute of Applied Chemistry, Chaoyang University of Technology, No.16, Jifeng East Road, Wufeng District, Taichung City – 41349, Taiwan (R.O.C).
K Mohanraj
Raman Research Laboratory, PG & Research Department of Physics, Government Arts College, Tiruvannamalai-606603, Tamilnadu, India
Kannagi Devendhiran
Department of Chemistry, Sri Vijay Vidyalaya College of Arts and Science (Women’s college), Dharmapuri, Periyar University, Tamilnadu, India.
Saroj Adhikari
Department of Environmental Engineering and Management, Chaoyang University of Technology, No.16, Jifeng East Road, Wufeng District, Taichung City – 41349, Taiwan (R.O.C).
Mei Ching Lin
Department and Graduate Institute of Applied Chemistry, Chaoyang University of Technology, No.16, Jifeng East Road, Wufeng District, Taichung City – 41349, Taiwan (R.O.C).
DOI: https://doi.org/10.26524/sajet190804

Published 2019-02-08

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Abstract

Mitochondrial and lysosomal membranes are prominent membranes of cardiac cells and are the factors that determine membrane function in myocardial ischemia. In this study, isolation of mitochondria and lysosome from heart tissue under the control, isoproterenol (ISO) (8.5mg/100g) induced cardiotoxic rats and oral pretreatment with Z. armatum fruit (200, 400mg/kg body weight) treated rats. Further characterization of marker enzymes was done. A decreased in the activity of all the mitochondrial and lysosomal marker enzymes in ISO administered cardiotoxic rats when compared to control rats which indicate ISO decreased the stability of the membrane. Pretreatment with hydroethanolic extract of Z. armatum fruit to ISO induced rats significantly reverted these biochemical alterations near to normal. The possible mechanism for the protection of heart mitochondria and lysosome against oxidative damage induced by ISO might be due to quenching of free radicals and enhancing the action of marker enzymes.

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